Co-founder Jim Duffy said he was in discussions with a Scottish council that was considering providing funding for a centre he hopes to open this year.
Mr Duffy declined to provide details but noted: "I would love to open one in Aberdeen. I would love to open one in Inverness."
The former policeman said he had also had talks with a high-level entrepreneur who might back the new centre.
Entrepreneurial Spark has developed hatcheries in Glasgow, Ayrshire and Edinburgh, with support from entrepreneurs including Sir Willie Haughey, Sir Tom Hunter and Ann Gloag.
The founders hope to encourage hopefuls by providing a free programme of mentoring with office accommodation and communications technology supplied.
Entrepreneurial Spark recently selected 37 people with ideas for potential businesses to be the first "chiclets" at the recently opened facility in Edinburgh.
A year after the first centre opened in Glasgow, Mr Duffy said the programme had succeeded on several fronts.
"We have created more jobs than we thought. We have brought in seven-figure investments [in chiclets]. We have got a new swathe of start-ups working with each other and networking."
Mr Duffy said 157 people had joined the programme to date. Some 57 had completed it. Around 80% of those who completed the programme are still training. Five or six have achieved six-figure sales.
Twelve found their plans were not viable or decided the programme was not for them.
The programme is delivered in association with Glasgow Caledonian and Napier universities and supported by commercial heavyweights including Royal Bank of Scotland.
Entrepreneurial Spark announced PwC had agreed a significant sponsorship programme. The accountancy giant will provide a five-figure funding package and workshops in business planning.
Lindsay Gardiner, head of PricewaterhouseCoopers in Scotland, said entrepreneurial businesses were an important cog in Scotland's economic engine. He added: "I am delighted PwC is joining forces with ESpark to help nurture and develop the next generation of entrepreneurs here."
Mr Duffy has been approached by several local authorities in England and Wales that want to learn more about the Entrepreneurial Spark model.
The organisation may roll the programme out through franchising or by admitting chiclets from south of the Border.
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