The company said it has launched a "major recruitment push" across the Central Belt as part of its efforts to beef up its human resources in areas such as web development and sales and marketing.
The recruitment drive is being launched in response to surging demand for the online services provided by Skyscanner.
Led by chief executive and co-founder Gareth Williams, the company expects to double revenues in 2013.
Mark Logan, chief operating officer, said more than 20 million people around the world are using Skyscanner to find flights, car hire and hotels each month.
Turnover grew by 65%, to £33.5 million in 2012.
The success could pave the way for a big pay day for shareholders should they decide to realise their investments in the business, which launched its website in 2001.
Mr Williams founded the business with Bonamy Grimes and Barry Smith. The three men hold a majority of the company's shares between them.
Scottish Equity Partners acquired a minority stake in return for an initial investment of £2.5m.
The new recruits in Scotland will be based in Skyscanner's headquarters in Edinburgh or in Glasgow, where the company plans to move its operations to a bigger office.
The company will hire a total of 100 new recruits for its offices in Singapore and Beijing and a new US centre it plans to open in Miami.