The £11 million, six-storey Clydeside hotel will create 65 new full- and part-time jobs for the city, including four apprenticeships,
Sited next to the BBC and STV headquarters and opposite the Hydro arena and SECC, the development also contains a 180-seater restaurant - the company's largest in Scotland - on the river.
Other facilities include two meeting rooms with views over the Clyde, self-service check-in facilities, free car parking and sheltered cycle parking.
Barry Halfpenny, Premier Inn Glasgow general manager, said: "Opening in time for the Games has been our aim since plans were first approved for the site, which everyone involved in the project has worked tirelessly to make happen.
"The hotel is in a fantastic location with incredible views both of the city and along the river which we are confident not only business and leisure travellers will be impressed with but locals too."
The Pacific Quay hotel will take the number of Premier Inn bedrooms to more than 1000 in Glasgow city centre, covering George Square, Charing Cross, Buchanan Galleries, Argyle Street and City Centre South (Ballater Street).
Pacific Quay is one of three Premier Inns being opened in Scotland over a 12-month period, along with another hotel in Stirling and a site in Perth.
A report by analysts BDO last month found that, for Glasgow hotels, rooms yield in January was up by 15% on the same month of last year at £34.90. Occupancy in the Glasgow hotel sector came in at 63.8%, up from 59.2% in the same month of 2013.