Bank of Scotland has teamed up with tourism marketing body VisitScotland to train a 12-strong team of relationship managers to better understand the needs of small independent and bed and breakfast hotels.
The training is understood to have placed particular emphasis on the challenges smaller tourism businesses face, including the seasonal nature of their earnings and their funding requirements.
Bank of Scotland plans to place the team in key tourist hubs around the country, including Inverness, Oban, Orkney and Aberdeen, as well as Glasgow and Edinburgh.
It launched the partnership with VisitScotland, which is believed to be the first of its kind, as the tourism industry gets set for a bumper 2014, when a series of major events will put Scotland on the world stage.
In addition to hosting major events next year, 2014 will also include a second edition of the Homecoming campaign.
That will see the country's food and drink and creative, ancestral and natural heritage promoted across a series of events.
Ian Collins, Bank of Scotland's area director for the south of Scotland, who is leading the tourism initiative, said: "This new tourism initiative will help build on the strong support we already provide the tourism sector by ensuring that we have a clearer understanding of the unique opportunities and challenges facing small tourism providers.
"This expert knowledge will enable our new specialist team to provide these businesses with the vital day-to-day support they need to grow and expand."
Mike Cantlay, chairman of VisitScotland, added: "The emphasis the Bank of Scotland is placing on investing in the future of one of the country's most important sectors is extremely encouraging.
"Now is the time for us to build and look to the future. The hard work does not stop in 2014, if anything it is the catalyst that will allow us to really put our stamp on the global tourism market for the latter part of this decade and beyond."