The brew, named Tennent's Beer Aged with Whisky Oak, has a 6% alcohol by volume content and will retail at £2.50 a bottle.
The company said the drink, which is made at its Wellpark Brewery in Glasgow, uses three types of locally sourced malt, a blend of four hop varieties and waters from Loch Katrine.
Fermentation sees it aged alongside single malt whisky-infused oak chips.
Ryder Cup venue Gleneagles and the luxury Torridon Hotel in Wester Ross and Inverlochy Castle Hotel near Fort William are among the places which will be stocking the beer. The Whisky Shop will also be selling the product online and in its stores.
Paul Condron, Tennent's marketing director, said: "Tennent's may be synonymous with lager, but Tennent's historically was the most successful ale brewer in Scotland. As the oldest and largest brewery in Scotland we feel we are in a great position to combine our beer heritage with the nation's other favourite drink, whisky. Reaction to the beer has been extremely positive both at home and in international markets."
Edinburgh independent brewer Innis & Gunn already has a number of beers aged in whisky, bourbon and rum barrels on the market.