Aberdeen was the best performer with occupancy rising by 11.5% and revenue by 23.6% to £66.92 boosted by several conferences in the city during the month.
Glasgow also reported increases with occupancy up 6.2% and revenue at £52.13, an 8.3% rise.
But there were dips in both measures across Inverness and Edinburgh.
However, overall Scotland outperformed the UK with occupancy growing 2.6% to 79.4% while room yield rose 5.5% to £52.33.
The survey by accountancy firm PKF showed a 0.4% growth in occupancy across the UK with revenue rising 2%.
Alastair Rae, a partner in the real estate and hospitality sector at PKF, said: "The overall figures for Scotland show a healthy improvement during October although these figures conceal widely differing performance levels."
Mr Rae added that unfavourable exchange rates and poor economic conditions appear to be restricting overseas leisure visitors and tourism revenue.