The Knowledge for Growth Initiative has guides, case studies, videos and webinars across a wide range of subjects.
Adrian Gillespie, managing director of operations at Scottish Enterprise, said: "With Scotland's economy now back on track to economic growth, it has never been more important to focus on supporting our ambitious companies to identify new opportunities for growth and attract investment.
"This new campaign is about doing just that. It's about raising awareness of the opportunities and information that's available to help Scottish companies grow and compete."
As well as advice from in-house staff at the agency, the initiative also provides the chance to learn from the experiences of business leaders across different industries.
The five main topics that have been identified are engaging in business overseas, accessing finance, innovating to develop new products and services, developing leadership and becoming more resource-efficient.
Kenny Richmond, economics director at Scottish Enterprise, believes companies need to adopt a 'global first' mindset and take more advantage of the opportunities available outside these shores.
He said: "Businesses in Scotland operate in a relatively small market. They should look outside Scotland, discover the overseas market.
"Companies which are operating there are performing better than those which don't. Those sectors doing particularly well - and identifying growth opportunities outside their normal comfort zones - are food and drink, and oil and gas."