Sarah Whitley and Matthew Brett, managers of the Baillie Gifford Japanese fund, held on to their joint first position when performance figures for the three years to August 31 were compiled by financial publisher Citywire.
The pair were ranked joint 23rd of asset managers in 35 countries whose performance is measured by Citywire, the highest ranking among those working for investment houses with a significant presence in Scotland.
Their Baillie Gifford colleague, Douglas Brodie, is placed sixth in the latest top flight.
Mr Brodie runs the Baillie Gifford British Smaller Companies fund.
In seventh place is John MacDougall, who runs the Baillie Gifford Japanese Smaller Companies fund.
Glen Finegan, who runs the First State Global Emerging Markets Leaders fund, climbed from fourth to third spot in the Scottish top flight when the latest performance figures were tallied.
His First State colleague Jonathan Asante, a one-time leader of the Scottish top flight, dropped from third to fifth place in the latest table.
Mr Asante is rated for his performance in managing funds including First State Latin America and First State Global Emerging Markets Leaders.
Edward Legget, who runs Edinburgh-based Standard Life Investments' UK Equity Unconstrained Fund, took fourth position in the Scottish top flight when the latest performance figures were calculated.
His colleague Lesley Duncan, who runs Standard Life Investments' UK Ethical fund, is in eighth position in the latest Scottish top table.
The Herald-Citywire survey measures risk-adjusted performance against relevant benchmarks. In total, about 100 fund managers working for investment houses with a significant presence in Scotland qualify for a Citywire rating, which denotes positive performance.
Of these fund managers, 21 are with Baillie Gifford and 12 are with Standard Life Investments.