Alastair Cumming, Clifford George, George Hunt and David Paterson are senior investment directors who manage money on a discretionary and advisory basis for clients including wealthy individuals and families.
They all worked for the venerable Gerrard stockbroking house before it was acquired by Barclays in 2003.
Tom Street, head of investment Management at Investec Wealth & Investment, said: "These appointments are further evidence of our conviction that Scotland offers good growth potential for our business.
"Respected investment professionals are attracted to the platform we have established over many years and the prospects we offer talented investment managers over the long-term. We are delighted to welcome our new colleagues in Glasgow."
The move comes just weeks after Investec, which has South African roots, recruited four senior investment managers from Deutsche Asset & Wealth Management to its Edinburgh office.
All four of its latest recruits began their careers with stockbrokers that through mergers and takeovers became part of Barclays.
Mr Paterson is a third generation stockbroker who began his career at Parsons & Co in 1986. He focuses on the private client work, dealing with trusts and self-invested personal pensions.
Mr George can also claim 27 years of industry experience after starting his career at Penney Easton. He focuses on similar areas to Mr Paterson.
Mr Hunt has 20 years of investment management experience having entered the industry with Parson Penny. He provides advisory and discretionary services and has clients ranging from families to pension funds.
Mr Cumming is senior investment manager with 35 years' experience of managing clients' assets and charity investments.
Mr Paterson will start work at Investec in December while his colleagues will begin in March.
Investec's Scottish presence was massively boosted by its acquisition of Williams de Broe in 2012.
Investec currently has nine employees in Glasgow and 48 in Edinburgh, before taking into account the new hires.
This is not the first time that Investec or its predecessors have looked to Barclays for recruits.
Williams de Broe established its operation in Edinburgh in 2008 with around 20 former Barclays staffers.
Barclays declined to comment.