Her comments at the state opening of parliament come with less than 500 days to go before the historic poll on the country's constitutional future.
Outlining the Coalition's programme today, she said: "My Government will continue to make the case for Scotland to remain part of the United Kingdom."
In the traditional ceremony, the Queen outlined eight Bills which apply in full to Scotland but none which apply exclusively north of the border.
It was also notable for what it missed out.