Ukip leader Nigel Farage said he "quietly" hoped the Queen backed Brexit.
Speaking ahead of a Grassroots Out rally in Newcastle, he laughed heartily when he was asked if Her Majesty favoured Brexit.
He replied: "I have spoken to a few members of the Royal family on social occasions.
"I don't know if Her Majesty favours Brexit.
"I'm certain she will say absolutely nothing during the course of this campaign.
"She has been the monarch for 64 years, what she says in private and what she says in public are two very different things.
"But I quietly, quietly, hope she does."
Mr Farage was due to share a platform with other politicians from across the political divide at the Tyne Theatre in front of 800 supporters, an organiser said.
Pro-Brexit Corby Conservative MP Tom Pursglove said someone had probably been "a bit naughty" in suggesting the Queen agreed with the campaign.
Prior to the rally, when he was asked if Her Majesty backed Brexit, he said : "I don't know and I would never want to speculate.
"We all got told off for that in Parliament.
"There are lots of people campaigning on both sides of the argument, I have no idea what Her Majesty thinks on this.
"I think someone has probably been a little bit naughty suggesting the Queen has a view on this one way or the other."
Why are you making commenting on The Herald only available to subscribers?
It should have been a safe space for informed debate, somewhere for readers to discuss issues around the biggest stories of the day, but all too often the below the line comments on most websites have become bogged down by off-topic discussions and abuse.
heraldscotland.com is tackling this problem by allowing only subscribers to comment.
We are doing this to improve the experience for our loyal readers and we believe it will reduce the ability of trolls and troublemakers, who occasionally find their way onto our site, to abuse our journalists and readers. We also hope it will help the comments section fulfil its promise as a part of Scotland's conversation with itself.
We are lucky at The Herald. We are read by an informed, educated readership who can add their knowledge and insights to our stories.
That is invaluable.
We are making the subscriber-only change to support our valued readers, who tell us they don't want the site cluttered up with irrelevant comments, untruths and abuse.
In the past, the journalist’s job was to collect and distribute information to the audience. Technology means that readers can shape a discussion. We look forward to hearing from you on heraldscotland.com
Comments & Moderation
Readers’ comments: You are personally liable for the content of any comments you upload to this website, so please act responsibly. We do not pre-moderate or monitor readers’ comments appearing on our websites, but we do post-moderate in response to complaints we receive or otherwise when a potential problem comes to our attention. You can make a complaint by using the ‘report this post’ link . We may then apply our discretion under the user terms to amend or delete comments.
Post moderation is undertaken full-time 9am-6pm on weekdays, and on a part-time basis outwith those hours.
Read the rules here