Ruth Davidson and Kezia Dugdale have said they will boycott The Open at Royal Troon if it does not overturn its all-male membership policy.
The Scottish Labour and Conservative party leaders told The Times that they would not attend the event in July if the club continued to exclude women from membership.
The Ayrshire club, which hosts The Open this year, is currently reviewing its membership policy. However, members will not vote on whether to open membership to women until after the tournament finishes.
Read more: Men-only Open golf venue should 'come into 21st Century'
First Minister Nicola Sturgeon has criticised the club for its male-only policy, however, she has not said she will not attend The Open over the issue.
She told The Times: "I'm the first woman to hold the office of first minister and gender equality matters to me. I don't think it's acceptable for there to be anywhere in Scotland that is off-limits to women and I hope Royal Troon reflects on that."
The intervention came after Maureen McGonigle, the founder of Scottish Women in Sport Foundation, urged Royal Troon 'to come into the 21st century'.
Former First Minister Alex Salmond MP boycotted The Open in 2013 over Muirfield's all-male membership policy.
Read more: A stooshie on the gender debate during Open week at Royal Troon will be a backwards step
Members of the St Andrews' Royal and Ancient Golf Club, the game's governing body, voted to end its all-male membership policy in 2014.
Stephen Anthony, secretary at Royal Troon, said: "The review is ongoing.
"We have to give due process to what we have put in place. It's a wider issue than just the gender issue at Royal Troon. It's not that simple."
Read more: Royal Troon's Postage Stamp to have Open Championship's 'wire camera'
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