Players Michael Boateng and Hakeem Adelakun, both 22, along with Chann Sankaran, 33, and Krishna Sanjey Ganeshan, 43, are accused of taking part in a conspiracy to defraud bookmakers.
Conference South players Boateng and Adelakun, both from Croydon, have been sacked by Brighton club Whitehawk FC.
It is alleged that with Ganeshan, 43, from Singapore and Sankaran, 33, from Hastings, they conspired to influence football matches.
The conspiracy is alleged to have taken place in Manchester and elsewhere between November 1 and November 26 this year.
Boateng and Adelakun both appeared on unconditional bail.
Sankaran and Ganeshanwere remanded in custody.
The four will next appear at the same court to enter their pleas on March 7 next year. A provisional date for a trial has been set for May 19, 2014, with the case expected to take up to four weeks to hear.