Stephen Craig, 35, from Clydebank, West Dunbartonshire, was jailed for three years and four months in October last year for arranging travel, accommodation and advertising for 14 women.
His co-accused, Sarah Beukan, who is known as Ashleigh Beukan, was jailed for a year-and-a-half for her part in the human trafficking scheme. Beukan, from Leith in Edinburgh, was 22 at the time.
In the first case of its kind in Scotland, the pair admitted moving 14 people to various addresses in Glasgow, Edinburgh, Belfast, Cardiff and Newcastle to work as prostitutes.
They were the first people to be convicted in Scotland under new legislation covering trafficking within the United Kingdom.
Craig is estimated to have made almost £250,000 in the six years before his arrest, but at Glasgow Sheriff Court yesterday a confiscation order for £45,000 was made against him.
Lindsey Miller, head of the serious and organised crime division of the Crown Office and Procurator-Fiscal Service, said it represented the "full amount available to us at this time".
"Stephen Craig took part in a criminal prostitution operation that spanned the United Kingdom," she said.
"His conviction, along with that of his co-accused Ashleigh Beukan in September last year, was a landmark case for human trafficking in Scotland.
"By virtue of this conviction, Stephen Craig was deemed to have committed a lifestyle offence under the Proceeds of Crime Act.
"This meant we could ask forensic accountants to look at all of his income during the six years leading up to his arrest .
"It was calculated he benefited to the sum of £236,651 in that period.
"Today's confiscation order for £45,000 represents the full amount which is available to us at this time from Stephen Craig."
Detective Inspector David Perrit, of Strathclyde Police's major crime and terrorism investigation unit, said: "Today's confiscation order sends a strong message that crime does not pay and clearly shows those who think they can make money from their criminal activities that the law will catch up with them."