The South Glasgow Hospital campus is due to open early next year and the project - the biggest of its kind in Europe - will cost £842 million.
But residents fear that 3,500 parking spaces, a large chunk of which may not be available at the time of opening, are not enough to accommodate around 10,000 staff and patients.
They also say 50 new promised bus routes, agreed at the planning stages, have "mysteriously evaporated" from the blueprint.
Campaign group the Health Service Forum South East fear this will cause chaos and misery for local residents as cars spill out onto their streets.
Patients, facilities and staff will be transferred to the new hospital from the old Southern General, the old Victoria Infirmary, the Western and the Royal Hospital for Sick Children at Yorkhill, which will then close.
A spokeswoman for NHS Greater Glasgow and Clyde said it was investing over £30 million in car parking facilities, including some multi-storey car parks and improvements to existing parking facilities.