He said he was looking forward to the venue being a "fixture" in Scottish tourism at the start of a week of events celebrating the industry.
Murray is preparing to launch the five-star Cromlix House Hotel, close to his home town of Dunblane, Stirling, next month.
The country hotel was the venue for his brother Jamie's wedding in 2010.
A message from the British number one, who is currently competing in the BNP Paribas Open in California, was relayed to hundreds of delegates at a conference in Edinburgh marking this year's Scottish Tourism Week.
Murray said: "2014 will be a big year - not only will I defend my Wimbledon title, I have joined your industry and now champion your message, that tourism is everyone's business, even tennis players'!
"2012 and 2013 were amazing years for me, winning gold at Wimbledon and then the slams in the US and at Wimbledon.
"But as an athlete, to stay at the top to be able to compete with the best, I know I can't sit back and there is always more to be done and I am very aware that you, the professionals in tourism, apply the same approach.
"On April 1 my hotel, Cromlix House Hotel in Kinbuck near Dunblane, will open to the public and that's me in your world and a fixture of the tourism industry in Scotland.
"Am I mad, I hear you say? No, just excited! My family are too, the hotel holds some special memories for us and the Dunblane community.
"I love Scotland, especially the people, the great food, the countryside and this new venture gives me the chance to do my bit to show off a bit of Scotland to the world."
The conference, organised by the Scottish Tourism Alliance (STA), aims to explore the issues facing tourism in Scotland and highlight opportunities for the sector.
STA chair Stephen Leckie said: "Scotland really does have it all, including a thriving tourism industry of which we can all be proud.
"We must now work together to join all the pieces of our wonderful jigsaw together and build a lasting legacy for the Scottish tourism industry."