The studio behind World War Z has paid more than £2.5 million to land the coveted TV spot during the American Football game.
The 30-second clip, which will be screened in a commerical break during coverage of the game, features new footage of countries around the world falling victim to a zombie outbreak.
Pitt – who plays UN worker Gerry Lane in the film – is seen joking with his family before Glasgow, doubling as Philadelphia, comes under attack.
Hundreds of people are seen fleeing through the streets of Glasgow city centre during the clip.
The advert will be screened just after the National Anthem at the match between the San Francisco 49ers and the Baltimore Ravens as the build-up continues for the film's release this summer.
Producers generally have hopes of huge box office takings for films advertised during the famous game.
World War Z, based on the novel by Max Brooks, sees Pitt's character taking charge when the zombie apocalypse hits, and attempting to save what remains of civilisation.
A report released last year said the filming in Glasgow provided a £3.33m boost to the local economy.