The accident happened at around 11.30am yesterday on the A91 between the villages of Strathmiglo and Gateside in Fife.
The male cyclist suffered serious injuries and died at the scene, police said.
They said no further details were being released until his family was informed.
The road was closed.
Inspector Brenda Sinclair said: "This collision has tragically resulted in the cyclist losing his life, and we are keen to hear from other motorists who may be able to assist us in establishing exactly what happened."
Anyone with information can call police on 101 or Crimestoppers anonymously on 0800 555 111.