The 34-year-old has been working with Barry McGuigan for the past month to prepare for his debut on November 30.
Flintoff, who famously led England to victory against Australia in the 2005 Ashes, retired from cricket two years ago.
The star said: "This is an amazing opportunity to try a sport I love, to be tutored by a man I respect and, aged 34, the chance to be a professional sportsman again.
"It's a huge challenge – probably the biggest I have ever undertaken, especially in such a short timeframe."
The bout at Manchester Arena will air on boxing channel BoxNation.