Cyclists competing in the Road Race on August 3 will speed along Argyle Street and Buchanan Street on a 14km circuit starting at Glasgow Green.
Spectators will not require a ticket to view the race, which also takes in Kelvingrove Park in the city's west end.
Glasgow 2014 organisers also revealed the route for the Time Trial cycling event on July 31.
Male competitors will ride 40km and women 30km on a single loop course starting at Glasgow Green and taking in the countryside of East Dunbartonshire and North Lanarkshire.
The team said it was working with Glasgow City Council and Transport Scotland to minimise disruption on the race days, when temporary traffic changes will be put in place.
Chief executive David Grevemberg said: "Cycling is among the fastest and most thrilling events at Glasgow 2014 with the Road Race and Time Trial offering fans the chance to experience the excitement of the sport up close.
"Road Race riders will take in some of the best sites in Glasgow, as well as some challenging climbs, while those undertaking the Time Trial will hit the open roads and head out of the city in a challenging race to the finish.
"These competitions, along with the Marathon, are free events to watch without tickets and we would encourage everyone to cheer the athletes on and enjoy what will undoubtedly be a unique Commonwealth Games experience."
Alasdair MacLennan, president of Scottish Cycling, said: "Following on from the record-breaking success of the 2013 British Cycling National Championships, which were held on the same course, I am sure the Road Race will be a fantastic spectacle showcasing the best of Glasgow to the world whilst offering a true test to the riders, many of whom will be travelling directly from the Tour de France to compete.
"The Time Trial route has been designed to present the athletes with a truly sporting and testing course. This course will offer riders and spectators a variety of road types, surfaces and landscapes whilst also bringing a free Glasgow 2014 experience to the public in the neighbouring local authorities."