The designated "comedy carriage" on the Virgin Trains 2.30pm London Euston to Glasgow service on February 27 will see passengers entertained by host Bruce Devlin, plus Patrick Monahan, John Hastings, Damian Clark, Jojo Sutherland, Ray Bradshaw, John Gavin and Phil Nichol.
They will be travelling to the Glasgow International Comedy Festival, which runs from March 14 to April 5.
Festival director Sarah Watson said: "We've done gigs in airports, stations and on streets but this is the first time that we've tried a comedy set on a train.
"You need nerves of steel to get up on any stage but this is a particularly daunting proposition. Luckily we have some of the best names in the business so we're guaranteed some laughs on the journey."
The comedy carriage will have unreserved seating and entry will not cost more than the price of a train ticket.
Virgin Trains expects demand for the event to be high. For more information, go here
Those attending gigs at the festival are entitled to 20% off advance tickets to and from Glasgow with Virgin Trains.
Adrian Varma, of Virgin Trains, said: "We're always up for trying something a bit different at Virgin Trains and this is definitely one of the most unusual events we've been involved with yet.
"It's a great line-up to see in any venue and I'm really looking forward to seeing how they manage the space."