The A947 in Aberdeenshire was shut for five hours around a mile south of Fyvie following the four-vehicle pile-up at 8.03am yesterday. The man, who has yet to be named, is believed to have been the motorcyclist.
The 38-mile road from Aberdeen to Banff is known locally as an accident black-spot. In the past six years, the route has been the scene of more than 150 crashes - including 14 fatalities.
Aberdeenshire Council last year launched a study looking at ways to improve safety on the road.
Local councillor Jim Gifford said: "My heart goes out to the family affected by this terrible tragedy. Police confirmed the motorcyclist who died was in his 20s."
Councillor Peter Argyle, head of Aberdeenshire Council's infrastructure services, said: "I am very sad to hear the news of this fatal accident.
"The council is doing a great deal to improve the safety of the A947 for all those using it."