The Nice Way Code campaign, launched today by Transport Minister Keith Brown, asks people to make small changes to increase road safety and improve the experience of driving, cycling or walking for everyone.
The initiative asks drivers to give cyclists more space and overtake them with care, and to look carefully for pedestrians crossing, while cyclists are asked to obey red lights and not cycle on the pavement. Pedestrians are included too, with messages about not trying to cross the road while looking at a mobile phone.
The campaign, funded by Transport Scotland and initiated by Cycling Scotland, will use television adverts accompanied by a poster campaign to get its message across to the public.
Mr Brown said: "The Nice Way Code campaign seeks to build a culture of tolerance and patience between cyclists, motorists, pedestrians and all other road users across Scotland.
"While the numbers of road casualties in Scotland are at their lowest ever level, there is still simply no room for complacency.
"I believe this campaign will play its part in making Scotland's roads safer for all users in the future and raise awareness that road safety really is everyone's responsibility."
Superintendent Iain Murray, head of road policing for Police Scotland, said: "We have seen in recent weeks the tragic consequences of cyclists and pedestrians being involved in road traffic crashes and incidents and there is a responsibility on all road users to be prepared, act responsibly and be aware of others around them at all times.
"Police Scotland is committed to keeping people safe on Scotland's roads and will continue to support campaigns and initiatives which help meet that commitment."