The Scottish tennis star bought Cromlix, three miles from his home town of Dunblane, central Scotland, in February last year.
The Victorian mansion has been "extensively" refurbished and tourism chiefs believe the revamped hotel will prove to be a big draw for visitors from all over the world.
In a statement on the hotel's website, Murray said: "By re-establishing Cromlix as a leading luxury hotel at the heart of the Dunblane community we will be able to attract new visitors to the area, create a number of new jobs and focus on supporting other local businesses. I'm pleased to be able to give something back to the community I grew up in."
The 15-bedroom hotel has been renovated to show-off much of the building's original heritage, including its own private chapel and loch.
The accommodation features 10 bedrooms and five suites which overlook manicured grounds and tennis courts.
It also boasts a restaurant called Chez Roux, overseen by legendary French chef Albert Roux and run by executive head chef Darin Campbell.
The hotel is being managed for the British number one by Inverlochy Castle Management International (ICMI).
Norbert Lieder, managing director, said: "Cromlix and Chez Roux combine to truly offer one of the best hotel and restaurant experiences in the country. The interiors, service, food and attention to every detail of the customer journey will make sure Cromlix becomes as well known for its service as it is for its famous owner."
VisitScotland chairman Mike Cantlay said: "We are delighted that one of our greatest sportsmen, Andy Murray, has entered the tourism game and I'm sure he will be a terrific ambassador for the industry.
"The revamped Cromlix is bound to be a huge draw for visitors from all over the world, generating the kind of excitement that Andy creates on court.
"As a local boy, from nearby Callander, the hotel itself has a very personal, special place in my heart and to see such an amazing redevelopment is truly inspirational."
The hotel is already fully booked for the Ryder Cup, which is being staged a short drive away at Gleneagles later this year.
Murray last year became the first British winner of the men's singles title at Wimbledon since Fred Perry in 1936.