The men from Addiewell Prison in West Lothian can be heard on the recording, called Changing Key, with members of the SoundRoutes choirs, a group of community choirs in central Scotland.
Four tracks feature on the CD, including a medley of House of the Rising Sun and Amazing Grace.
There is also a version of The Who's Behind Blue Eyes, Primal Scream's Movin' On Up and Jimmy Cliff's Hard Road To Travel.
The CD was produced by flautist Shona Brown, who runs SoundRoutes.
It is being launched at a concert in Glasgow's Mitchell Theatre tomorrow, with all proceeds from the sale of the disc going to Shelter Scotland.
Ms Brown said: "The atmosphere was truly special and a memory I will treasure forever.
"I believe the prisoners have learned something new and positive about themselves.
"I hope this experience has been life-changing for everyone, if only in a small way."