Craig and Charlie Reid will play their hit, one of the late MSP's favourite songs, in honour of her lifelong love of Hibs FC. Their rendition, made all the more poignant as Ms MacDonald's daughter Petra is married to Craig Reid, will end a public memorial service at the Kirk's General Assembly Hall.
Friends, family and colleagues from the Independent Lothian MSP's long political career have been asked to wear bright colours at her request.
They will arrive on The Mound to the sound of some of her favourite country and western tunes.
Dr Bob Scott, a humanist celebrant, will introduce the main speakers, including Health Secretary Alex Neil and actress Elaine C Smith, both close friends. The final speaker will be Ms MacDonald's husband, Jim Sillars.
Ms MacDonald died earlier this month at the age of 70.