Peak times at the Glasgow attraction saw visitors having to wait around 40 minutes to view the Scottish artist's work. A total of 2500 people attended on Saturday, with larger numbers expected to have passed through the doors yesterday. The museum extended the opening times for the last day of the exhibition, giving the public until 8pm to view the famed artwork.
More than 130,000 visitors came to Kelvingrove for the 22-week run, making it the most visited art exhibition ever to go on display at the museum.
Victor and Colette Clews travelled from Birmingham to see the exhibition on its final day.
Mr Clews, 70, said: "We've been collecting some of his limited edition prints for about 12 years now. His work is always interesting.
Ms Clews, 50, added: "I have been looking forward to this for a long time.
"We have some of his work in prints - we absolutely love his work. To be honest, we didn't realise how big the queues were going to be, but I know it will be worth it.
"The time constraints of work, and travelling here, meant this was really the first, and only chance, we would get to see his work."
John Butcher, 31, of Ayrshire, said he had come to see the paintings after friends had recommended it. He said: "We are really here just to see the paintings and learn a few of the stories behind them."
Jim Murphy, 63, from Dumbarton, added: "I come to quite a few exhibitions, and I'd say this is one of the busiest I've been to.
"All the large exhibitions generate good public interest, which can only be good for Glasgow as a whole."
Vettriano's success exceeded that of the Glasgow Boys exhibition, which attracted 123,289 visitors in 2010.
The artist, who recently moved back to Scotland from England, said: "I was astonished and very flattered to find out that my retrospective exhibition has received a record-breaking number of visitors.
"From the very outset, I have been treated with enormous warmth and appreciation by the management and staff of Kelvingrove Art Gallery and I am deeply touched to hear that their enthusiasm has been echoed by so many of the visitors to my exhibition, during its five-month run.
"I am also hugely indebted to the collectors of my work, who so generously loaned their paintings for this retrospective. Exhibiting in such majestic surroundings as Kelvingrove has been a great honour."