The £9.3 million revamp of the category A-listed structure in Midlothian, which was made famous by the film The Da Vinci Code, had to be carried out after a report warned of damage to the historic stonework because of dampness and high humidity levels.
A steel frame had been wrapped round the building since 1997 to allow the stone roof to dry naturally.
The small rural church became a world focus after the Dan Brown book that highlighted the mysterious symbolism of its ornate stone carvings. The novel, which was later turned into a film starring Tom Hanks and Audrey Tautou, has helped raise funds for the conservation project, aided by National Lottery and Historic Scotland grants.
The chapel - an Episcopal place of worship - was founded in 1446 and is privately owned by the Earl and Countess of Rosslyn.
The stonework has always attracted academic interest, but the publication of the book saw annual visitor numbers rise to 176,000.
Ian Gardner, director of Rosslyn Chapel Trust, said the scaffold "had become a near permanent feature".
"Visitors can now enjoy a clear and uninterrupted view of the exterior of the building, which, like the rest of the chapel, is rich in carvings and details," he said.
Fiona Hyslop, Culture Secretary, said Historic Scotland gave £1.6 million to the project to help fund the "painstaking" renovation.
She said yesterday that the site is unique, adding that the work "allows us to see the chapel in a whole new light".