All spectators will have to arrive by a dedicated bus or train, with no access for private vehicles during the event from September 23 to 28.
Access to Gleneagles station is being enhanced and a temporary footbridge will carry ScotRail passengers over the A9.
Two new temporary bus terminals are also being constructed to handle the flow of park-and-ride buses.
Access to and from Auchterarder will be kept open, but road restrictions will apply.
Locals are being informed of the proposed transport plan at a series of meetings taking place in Auchterarder, Kinross, Stirling and Perth this week.
Three park-and-ride facilities at Balado, Perth and Stirling are expected to cater for the majority of the thousands of golf fans attending.
Antonia Beggs, operations director at Ryder Cup Europe, said: "Our transport plans for the 2014 Ryder Cup aims to ensure that people can get on with their daily lives, whilst also help delivering one of the greatest sporting events in the world.
"We have tried to ensure that the event is as sustainable, safe and efficient as possible."
Laurence Kenney, from Transport Scotland, said: "Partnership has been key to the detailed planning undertaken to develop this transport plan which aims to ensure the reliable transport of thousands of spectators, while also keeping Scotland's major roads flowing. The three park-and-ride facilities, combined with enhanced rail provision, offer a number of options for spectators, while also minimising the impact on local communities."
Ian Miller, leader of Perth & Kinross Council, said: "We want all spectators to enjoy their visit to Gleneagles through using high quality, sustainable public transport provision. We are confident that the transport plan will help to deliver this, while ensuring that the majority of local people can continue with their daily lives as normal."