Inverness Caley Thistle, Aberdeen and St Jonstone joined the growing ranks of clubs who said they would reject an application from Charles Green's club to join the Clydesdale Bank Premier League next season.
The three SPL clubs confirmed their opposition following similar moves by Dundee United, Hearts and Hibernian.
With six clubs opposing the move, Green's club is consigned to starting life outside the top flight.
There was no immediate reaction from Rangers. The main question facing Green now is whether he can secure a deal to demote Rangers to the Irn-Bru First Division amid talks aimed at restructuring the Scottish game or whether he will be forced to apply to gain entry to the Third.
A statement from the Highland club read: "Inverness Caledonian Thistle FC has completed their consultation exercise and consideration of its options in relation to the upcoming SPL vote on July 4.
"The club will be voting 'no' to an application for SPL entry from a new company which has acquired the assets of Rangers following the liquidation of the previous company.
"Having considered all the options and following a very productive discussion with the supporters trust board, the ICTFC directors are unanimous in their decision."
Within minutes of the announcement, Aberdeen became the fifth club to confirm they will vote against a newco Rangers joining the SPL. St Johnstone added their opposition soon afterwards.
Aberdeen chairman Stewart Milne said in a statement: "Traditionally we have preferred not to make public our voting intentions, but in light of the level of interest and the fact other clubs have chosen to show their hand, on this occasion I can confirm it is our intention to oppose readmission to the SPL for any Rangers newco.
St Johnstone's statement read: "Notwithstanding the potentially damaging financial implications, the board believes that sporting integrity should not be sacrificed in favour of economic expediency.
"Over the last few weeks there has been a mounting groundswell of opinion amongst our fans which has clearly shown that the vast majority of our supporters would not favour an application by newco Rangers to the SPL. This has simply reaffirmed the board's position.
"It is important that a clear and unambiguous message goes out from the custodians of Scottish football that this sort of scenario should never be allowed to happen again.
"As a result, St Johnstone Football Club will not support an application by newco Rangers to join the SPL. We would urge all St Johnstone fans to get behind the club and offer their support in the challenging season which lies ahead."
Caley Thistle chairman Kenny Cameron said: "The past few weeks have been extremely hectic in terms of our supporters and season ticket holders making their views known in great numbers to the club, as is their right.
"It is fair to say that in excess of 95% of them raised the issue of sporting integrity as the reason why they don't want the newco to be admitted and had not yet renewed their season tickets.
"Financially, we examined the various scenarios and, frankly, none of them were positive for our club... it is extremely disappointing to be adversely affected by something not of our doing, unfortunately this is a similar situation for the rest of the clubs in SPL.
"We have also been contacted by supporters of various other SPL clubs, saying they would not be back to Inverness if we did not accept that sporting integrity was what mattered, but this is something that we had already considered and it is not only the integrity of the SPL that is at stake but the integrity of Scottish football.
"It is quite clear that supporter opinion in Scotland overwhelming supports that view. Supporters are the life blood of any club and we would have been foolish not to take on board their opinions on this matter, they are our customers and any business that ignores the views of its customers does so at its peril.
"A substantial amount of our own fans have withheld from buying their season tickets, saying they will not do so if we vote for a newco to re-enter directly in to the SPL. In now making clear how we will vote, we expect these fans to come forward fairly quickly and renew their season tickets and give Caley Thistle the financial injection it requires for the 2012-13 campaign. We are hoping that all the ICT fans will now rally round and buy extra season tickets to cushion the blow of the potential loss in revenues from elsewhere."
Cameron added: "We would be in a completely untenable position, financially and morally, if we defied the overwhelming demand of our own paying customers who are, after all, what Caley Thistle are all about."
Elsewhere, Motherwell have entrusted members of their fans' ownership group with the decision, while St Mirren have launched a formal consultation with supporters.
Kilmarnock's Michael Johnston is the only chairman to make clear his support for Green's bid but the Ayrshire club subsequently launched a consultation exercise with fans.
More than 1,000 fans who have pledged money to St Mirren's 10,000 Hours community ownership scheme will be consulted on the club's decision but, unlike Motherwell, they will not get a yes/no vote.
The club last week announced they had postponed a sale of the majority shareholding until the commercial implications of the Rangers situation became clear. Saints fans have been given a list of possible financial scenarios resulting from a newco being excluded and have been asked whether they would be prepared to support the club with increased donations should commercial revenues fall.
The consultation document said: "Depending on the funding required, this may delay the purchase for a year with your monthly contribution going towards helping the club directly while helping to maintain the integrity of Scottish football."
Contextual targeting label: