The duo are riding high in terms of popularity following the release of the film blockbuster Sunshine on Leith, a movie based on the band's music and named after their best-known album.
Identical twins Craig and Charlie Reid formed the band in 1983, and have risen through the ranks to become a household name and one of the nation's biggest bands over the last 30 years.
Announced as their last concert before 2015, more than 9000 fans travelled far and wide to watch as they played a two-hour set at The Hydro, supported by Glasgow band Glasvegas. Read our review
Julianna Moats, 27, is originally from California and has seen the band perform more than 120 times. Now living in Birmingham, she once travelled more than 15,000 miles from her hometown to see them play, often clearing her bank account to avoid missing a show. Speaking outside The Hydro, she said: "They are my favourite band in the whole world.
"It's the biggest show tonight and it's the last one of the tour so I have to be there. There is a Proclaimers song for everything in life and they will never hold back or compromise for what they believe in."
Recently released blockbuster Sunshine on Leith brings the band's songs to life on the big screen and could help to propel them into superstardom, having taken more than £1.4 million at the box office in its first week.
It has been likened to films such as Mamma Mia for its feel-good plot set to a backdrop of Proclaimers tunes, including the classic anthem I'm Gonna Be (500 Miles).
Starring the usually ferocious Scots actor Peter Mullan, it was adapted from the successful stage version of the story created by playwright Stephen Greenhorn in 2007 and tells the story of two soldiers trying to deal with normal life in Edinburgh as they return from duty in Afghanistan.
Many of the fans at last night's gig weren't there because they'd been inspired by the film, but simply because they wanted to see the Scottish stars in the flesh and experience a concert in the new venue.
Superfan Moats is glad the release may help to bring in a new generation of fans, but hopes the brothers will still remain grounded if they do become more popular around the globe.
"I really hope the film will help them to reach a bigger audience and become more famous, but I hope not too much. One of the reasons why I still love to come to see them is because they are still so down to earth.
"They always give a great show, they never just phone it in and they're really nice guys as well."
The Hydro gig is the furthest fan Lynn Thompson from Blackburn has ever travelled to see the duo play, and she has loved them since hearing their first album, This is The Story, which was released in 1987.
"Their albums are getting better and better as they get older," she said. "I'm really excited about seeing them tonight. From young to old, their music appeals to everyone and they've got a song for everything."
Kathleen Heraghty regularly goes to gigs around Glasgow with a group of friends and particularly enjoys the band's lyrics, which tend to speak about politics and everyday problems.
Heraghty, 55, from Clydebank said: "I like their music and a lot of their songs have great lyrics, and they mean a lot to so many people in Scotland.
"I've always liked the fact they sing in their own accents. In its place I think it is fine for people to go more Americanised but for what they write it suits them; the music and lyrics wouldn't sound right without a Scottish accent."
Younger fans Jamie-Lee Syme from Falkirk and Steven Derry from Grangemouth were even more excited about the concert after seeing Sunshine on Leith, and were looking forward to trying out The Hydro as well.
Derry commented: "I like their music, and the fact they're Scottish.
"I remember them as a child, listening to them with my family - one of the first songs I ever remember listening to was Letter from America and I think they're great.
"Watching the new film was fantastic as well but I'd have liked to see them more in the film."