The 28-year-old was pictured carrying out manoeuvres across the apex of the 174ft (53m) tall structure on the banks of the River Clyde as part of a promotional video for the Glasgow 2014 Games.
The rider was filmed on top of the disused crane as part of a series of short films being produced by the BBC at various locations in Glasgow that are due to be shown during the Commonwealth Games.
The stunt cyclist was expected to do further filming for the three-minute video at Glasgow's Millennium Bridge.
Mr MacAskill, who is originally from Skye but now lives in Glasgow, shot to worldwide fame in 2009 after he posted a film of himself doing stunts on his bike on YouTube.
The video became a viral hit on the internet. Like the Commonwealth Games video, the YouTube stunts were performed to a backdrop of iconic Scottish landmarks, from Edinburgh Castle to the Forth Bridge.
Mr MacAskill had been practising his skills for more than 12 years before he gained fame overnight for his homemade video in April 2009. He gave up his job as a mechanic so he could ride full-time and now works professionally as a street trials pro rider for Inspired Bicycles Ltd. Since 2009, MacAskill has appeared in the music video for Doves' single Winter Hill, and in May 2011 Leica Camera released a Go Play promotional video featuring him doing tricks in Cape Town in South Africa.
In August 2011, MacAskill also featured in a video named Industrial Revolutions, which featured the stunt cyclist doing tricks in an abandoned Scottish iron works. It was created for Channel 4's documentary Concrete Circus.