Conservationists have launched a petition calling on the organisers of T in the Park to find a new venue.
Woodland Trust Scotland say the move is necessary to protect the trees and wildlife at the Perthshire site..
DF Concerts had to move the huge music festival from its original venue near Kinross, because of safety concerns over an oil pipe running underneath the field. Strathallan Castle Estate near Auchterarder was chosen, just 20 miles to the west.
But major environmental concerns have been raised particularly over the fate of two ospreys which are due to return to their nest nearby, any day now.
The festival organisers have built a new nest but have been unable to remove the existing one because it son land owned by an unnamed party who is refusing access.
The Woodland Trust claims the site is surrounded by 200 acres of important woodland which is home to iconic wildlife
The trust's Charles Dundas said: "The woodland at Strathallan Estate deserves protection. Holding T in the Park there will drive many rare species of wildlife, including bats, otters and ospreys away. We're not against T in the Park itself, however these woods are simply too important for wildlife for a festival of this scale to be held on just metres from its edge."
But a spokeswoman for T in the Park said "Once again, the Woodlands Trust has shown that they're more interested in a cheap headline than actually spending time reading our planning documents or taking part in the proper planning process. As has been widely reported, an addendum document with additional information on wildlife is being presented to Perth & Kinross Council and will then be available for public consultation for a further 28 days. Why wouldn't the Trust take the time to analyse this information and then submit their response like every member of the public is entitled to do?"
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