Liam Fee, who was two, was found dead at a property in Donald Crescent in the Thornton area of Fife on Saturday March 22.
At the time, police said his death was being treated as unexplained and an investigation into the circumstances has been ongoing since.
Two women, aged 30 and 27, have now been arrested in connection with the death and are due to appear at Kirkcaldy Sheriff Court later.
A Police Scotland spokesman said: "Two women have been arrested and charged in connection with the death of a two-year-old boy in Fife earlier this year.
"Liam Fee was pronounced dead at his home in Thornton on Saturday March 22 and a police investigation into the circumstances surrounding his death has been ongoing."
Liam was described as a "happy-go-lucky" boy by his family after his death.
A statement released through police at the time said: ''Liam was a happy-go-lucky little boy, with blonde hair, big blue eyes and a cheeky smile.
''He was very much loved by his family and will be deeply missed. Liam had his whole life ahead of him and we are devastated at our loss."