The fresh hope for a cub came after it emerged the panda had been pregnant but had lost the unborn infant and a zoo spokes-man said they would be "trying again next year".
News on the pregnancy has been awaited for weeks but, despite staff doing everything possible for Tian Tian, the miscarriage was confirmed yesterday.
A new round of tests to determine when to put Tian Tian and her male partner Yang Guang together again so their hormone cycles match could begin as early as January. This year the pair were put together in April after such tests but failed to mate naturally.
The Royal Zoological Society of Scotland, which runs the attraction, confirmed there had been a pregnancy after artificial insemination but the unborn cub had not survived.
Iain Valentine, the director of the panda project at the zoo, said it is trying to establish what had happened and when.
He said: "What we can say is that when we look at all the data she did conceive, she did get pregnant, and what we're trying to do now is pinpoint where she may have lost her pregnancy.
"That is quite tricky to do with the information we have got.
"Why does this happen? We don't truly understand, but what we do know is this is a species which lives on the edge of a balance between nutritional intake and reproducing.
"If things aren't quite right this can be a reason why they absorb the young and cancel the pregnancy.
"We are determined to learn from this experience and if we can improve then we will do."
Zoo chief executive Chris West said: "We are all saddened by this turn of events after so many weeks of waiting.
"Such a loss has always been in our minds as a real possibility, as it occurs in giant pandas as well as many other animals, including humans."
Mr West added: "We are conducting a detailed review of the scientific data collected, but I am totally confident that we did everything it was possible to do.
"The majority of research centres and zoos with giant pandas around the world have not successfully bred until the third or fourth year and what we have achieved considering we have had giant pandas for less than two years is immense."
The pair arrived in Scotland in December 2011 under a 10-year deal costing £6m a year. The adults have already boosted attendance and it has been suggested a cub could bring in £50m to the Scottish economy.
Tian Tian was inseminated on April 21 using semen from Yang Guang and frozen semen from Bao Bao, a "genetically important" panda which died in Berlin Zoo last year.
If a cub had been produced it would have been the property of China and would have been returned there after two years to join a conservation programme.