The incident happened during the evening rush-hour on Wednesday on the southbound carriageway of the A9 at Dunblane.
A 47-year-old was driving his Ford Transit van when he was in collision with the back of a Volvo flatbed lorry which was queuing in traffic. Police, fire crews and paramedics were called to the scene where the van driver was pronounced dead.
The road was closed for six-and-a-half hours in both directions.
The crash happened at around 5.45pm, about 600 yards from the Keir roundabout.
Police are asking anyone with information about what happened to come forward.
Sergeant Alistair MacLean, of Police Scotland's Forth Valley division, said: "Tragically, this incident has resulted in the van driver's death and our inquiries are continuing to establish exactly what happened. We are asking for motorists who were on the road and witnessed the collision to come forward immediately."