Mladic, 70, sat listening with his back to the public yesterday after being warned on Wednesday for making a throat-slitting gesture to a relative of victims.
The massacre, Europe's worst atrocity since the Second World War, helped finally to galvanise Western powers into launching air strikes on Serb forces to bring the 1992-95 Bosnian War to an end.
"This was and will remain genocide," said prosecutor Peter McCloskey, showing grainy video footage of bodies outside a warehouse where about 1000 prisoners were shot. "The evidence of this crime is overwhelming. We will focus on linking General Mladic and his men to the crime."
However, there was a blow for efforts to ensure the trial of Mladic – whose lawyers claim has had three strokes and a heart attack – does not parallel that of former Serb president Slobodan Milosevic, which lasted so long he died before a verdict was reached.
Mladic looks frail and thin compared to the stocky general seen barking orders to shell Bosnian Muslim positions, but has benefited visibly from the medical treatment he has received while in detention.
The judges accepted a defence argument that prosecutors had not disclosed their case properly, but did not say if they would grant the full six-month delay requested by the lawyers before the trial enters its next stage, where evidence is presented.
Mr McCloskey said prosecutors planned to call scores of witnesses, including 11 survivors of the massacre as well as executioners from the Bosnian Serb army.
"In only five days, forces of Radovan Karadzic and Ratko Mladic expelled the population from Srebrenica and Zepa and murdered more than 7000 Muslim men and boys."
He said nearly 6000 bodies had been exhumed from mass graves and secondary sites where bodies were reburied to conceal them in remote mountain areas. Their remains have been identified by DNA testing.
In the public area, mothers of Srebrenica victims wept as they listened to the proceedings.
A relative, Zumra Sahomerovic, said: "My husband was 45 years old. He was taken away and killed only because he had a different name and different religion."
Another relative, Hatidza Mehmedovic, wept in the court's lobby during a break in the proceedings. She said: "I buried both of my sons and my husband. Now I live alone with memories of my children. I would never wish even Mladic to go through what I go through."
The prosecution claims the massacre was part of a plan to "cleanse" parts of the Balkans of non-Serbs and create a pure Serb state.
Among the 11 charges against Mladic are genocide, murder, rape, imprisonment and acts of terror for actions that also include the 43-month siege of the Bosnian capital, Sarajevo, in which 10,000 died, and the establishment of a number of brutal prison camps.
Like former Bosnian Serb leader Karadzic, who is also on trial in The Hague, Mladic faces life imprisonment if found guilty. The former general is held in a one-man cell in a special international wing of a Dutch jail.
Both were indicted by the International Criminal Tribunal for the Former Yugoslavia in 1995, but evaded arrest for years before being tracked down.