A new programme on Scotland's constitution has become embroiled in a row over claims of political bias.
Labour peer George Foulkes has complained to STV and broadcasting regulator Ofcom about Herald columnist Iain Macwhirter presenting Road to Referendum.
However, Lord Foulkes said Ofcom declined to investigate the complaint.
Mr Macwhirter said Lord Foulkes would find himself in court if he continued to challenge his professional integrity.
The three hour-long shows, the first of which will be broadcast tonight, look at the history and development of the Scottish independence issue.
First Minister Alex Salmond and Prime Minister David Cameron are among the political heavyweights to have contributed to a show the SNP called "accessible and engaging".
Lord Foulkes is unhappy that Mr Macwhirter is the sole presenter. The former Labour MP and MSP said he complained earlier this year to STV and Ofcom: "I wrote to STV and suggested they should have someone else alongside Iain presenting the programme.
"Iain is a good journalist but he holds a particular view on independence."
He added: "I think STV dealt with my complaint in a cavalier manner."
Lord Foulkes said Ofcom did not look into his complaint.
Mr Macwhirter, who used to be a member of the Labour Party, denied the accusation he was partisan. He said: "Anyone who reads what I have written knows that I am far from being a committed nationalist and I have never been a member of the SNP.
"If he doesn't cease challenging my professional integrity he is likely to find himself explaining his remarks in court."
A raft of Labour politicians were interviewed including Baroness Liddell of Coatdyke, Scottish party leader Johann Lamont, and former first minister Jack McConnell.
An SNP spokesman said: "STV is an independent broadcaster committed to impartiality, and Mr Macwhirter is a highly respected and objective commentator.
"This attempt to censor the media by complaining about a programme that hasn't even been screened yet is a new low for No campaign supporters."
An STV spokesman said: "As a public service broadcaster STV has a duty of impartiality.
"Road to Referendum has been subject to STV's established and thorough compliance process and the series is balanced and impartial."
l Former Labour chancellor Alistair Darling, who chairs the anti-independence Better Together campaign, is to speak at a fringe event of the Scottish Conservatives conference in Stirling on Saturday.
He will launch the Forces Together – a network of former UK Armed Forces Personnel and their families who believe that Scotland is stronger as a part of the UK.
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