RUTH Davidson has distanced herself from comments by David Cameron when the Prime Minister warned he would block a second independence referendum.
Mr Cameron told the told Westminster journal The House earlier this month that the independence question was "settled for a generation".
Asked what his position would be if the SNP won the next Holyrood elections with a manifesto commitment to a second referendum, Mr Cameron replied: "That issue is settled."
But the Scots Tory leader suggested a future Conservative government would respect a Holyrood vote for a second referendum.
Launching a new election broadcast in Edinburgh, she said: "The Prime Minister and I have spoken about this at length, most recently when we were launching our manifesto in Glasgow.
"There is an absolute precedent that has been set down and I do not deviate from that precedent."
Asked if she would advise David Cameron to block another referendum, she said: "Absolutely not.
"We have and have always believed in self determination and democracy, and we have just had the largest democratic event in the history of our country, 85 per cent turnout, which has never been seen not just in Scotland, but in any democratic event anywhere in the United Kingdom.
"We voted to stay part of the UK and we were told that that would be it for a generation.
"The best way to stop a second referendum is to make sure that you vote for parties other than the SNP in the Scottish Parliament next year in 2016."
Ms Davidson said people should "vote for what you believe in," a message designed to dissuade Tories from voting tactically for other parties.
She said supporters should get out and "evangelise" for the Conservative cause.
"The shy Tory syndrome that Scotland has suffered from before, it shall suffer from no longer," she said.
"We are proud of our achievements in government and we are proud of our achievements in the referendum.
"We know what we stand for, we speak our truth loudly, we speak it clearly and there are people across Scotland who want to listen to us again."
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