The appeal was lodged against the sanctions imposed on the Liverpool striker by FIFA's disciplinary committee for biting Italy's Giorgio Chiellini. They included a nine-match international ban plus a 100,000 Swiss frand (£66,000) fine.
FIFA head of media Delia Fischer told a news conference in Rio de Janeiro: "The FIFA appeal committee has decided to reject the appeals lodged by both the Uruguayan player Luis Suarez and the Uruguayan FA, and to confirm the decision rendered by the FIFA disciplinary committee on June 25 2014 in its entirety.
"The terms of the decision taken by the FIFA appeal committee were communicated to the player and the Uruguayan FA today."
Both the player and the AUF can make a further appeal to the Court of Arbitration for Sport.