As well as lifting the other race run over the Grand National fences with Little Josh, they also struck with Master Of The Sea.
But it was 14-year-old Hello Bud (14-1), who took the plaudits with an emotional triumph for his connections. Successful in 2010 and running in this race for a fourth time, the veteran answered his rider's every call.
Join Together was clawing back the deficit with every stride from the elbow but was still a neck down at the line.
The winning jockey said: "He absolutely ran his heart out for me. He means everything to me. I just can't tell you just how much I love this horse. He's given us so much pleasure over the years."
While his father seemed keen for Hello Bud to keep going, Twiston-Davies jnr feels it would be fitting for Hello Bud to retire on such a high.
He said: "I think it was the perfect way for him to go out today and I'd like to think he's finished. I know he can run in the National but it would be shame to keep dragging him on."
Twiston-Davies became the first to train winners of all five races run over the National fences when Little Josh (7-2 favourite) landed the Betfred Goals Galore Grand Sefton Handicap Chase, beating Gansey by a length-and-three-quarters.
At Sandown, Sprinter Sacre's easy success in the Sportingbet Tingle Creek Chase was the highlight of a four-timer for trainer Nicky Henderson, who also teamed up with stable jockey Barry Geraghty to claim the Markel Insurance Henry VIII Novices' Chase with Captain Conan.
Sprinter Sacre (4-11 favourite) extended his unbeaten run over fences to six on his seasonal bow in the Grade One feature.
Much had been made of his showdown with the Paul Nicholls-trained Sanctuaire but the latter only managed third place after being caught by Kumbeshwar on the way to the line.
Paddy Power now make Sprinter Sacre the 1-2 favourite from evens for the Queen Mother Champion Chase at Cheltenham.
Henderson's other wins came courtesy of Petit Robin (11-1) in the Sportingbet Handicap Hurdle and Golden Hoof, in the opening John Oakley 50th Birthday "National Hunt" Novices' Hurdle.