Green-Tregaro competed in Thursday's qualifying round of the high jump at Luzhniki Stadium with her fingernails painted in the colours of the rainbow flag used by the gay movement.
"It's disrespectful to our country, disrespectful to our citizens because we are Russians," Isinbayeva, speaking hesitantly in English, told a news conference. "Maybe we are different than European people and people from different lands. We have our law which everyone has to respect. When we go to different countries, we try to follow their rules. We are not trying to set our rules over there. We are just trying to be respectful.
"We consider ourselves, like normal, standard people, we just live boys with women, girls with boys . . . it comes from the history. I hope the problem won't ruin our Olympic Games in Sochi ," added the 31-year-old, who is one of Russia's best-known athletes and won her third world title on Tuesday.
The controversial Russian legislation, which was passed in June, outlaws some aspects of the promotion of homosexuality and has become a political hot potato ahead of next year's Sochi Winter Olympics, when it will apply to athletes and spectators.
Critics of the law have said it effectively disallows all gay rights rallies and could be used to prosecute anyone voicing support for homosexuals.
Last week, the International Olympic Committee (IOC) said it had sought clarification from Russia on how the law would be applied while there have already been some calls for a boycott of the Games.
Isinbayeva, who is an ambassador for Sochi 2014 and will be mayor of the main athletes' Olympic village, was against this. "Of course, I don't support this boycott," she said. "I'm also against this polemic and feel sorry that they try to involve the athletes in such a problem . . . because we are not prohibiting athletes from participating in Sochi even if they have no traditional relationships."