The British No.1 has confirmed he will defend his title at the AEGON Championships, which begin on June 9.
Murray is currently tied with Jimmy Connors on three titles and will bid to join John McEnroe, Boris Becker, Lleyton Hewitt and Andy Roddick in lifting the trophy for a fourth time.
Last year Murray beat Croatia's Marin Cilic in the final before going on to become the first British man in 77 years to win the Wimbledon singles title three weeks later.
Murray, who also won at Queen's in 2009 and 2011, said: "To win the title for the third time last year and then to go on to win Wimbledon was really special, and it highlights the importance of Queen's.
"The grass courts there are as good as any in the world and it's a great event. It's also a tournament with a lot of history, both personally for me and for the game as a whole.
"It's where I won my first professional match and my first title in Britain and, when you look at the names on the trophy, including my coach Ivan Lendl, it shows how big a tournament it is to win. I'd love to do it again."
Last year's final was extra special for Murray because, following the match, he took part in the Rally Against Cancer to raise money for the Royal Marsden hospital, where his friend Ross Hutchins received treatment.
Murray donated his winnings of £73,000 to the cause, and said: "It's a day I'll never forget."
British Davis Cup player Hutchins was diagnosed with Hodgkin's lymphoma, a form of cancer, in December 2012 but is now in remission and returned to the ATP Tour at last week's Brisbane International.