Leaving the European Union will cost Britain billions of pounds in infrastructure investment, David Cameron has warned.
The Prime Minister said a vote for Brexit would mean an end to Britain's membership of the European Investment Bank (EIB) which has poured more than £16 billion into UK projects in the past three years.
Ian McConnell: Brexit arguments sublimely ridiculous as economic realities ignored
He said that withdrawal from the EIB would have a "devastating impact" on future infrastructure projects.
"Vital projects across every region of the UK have been financed by the EIB. These make a huge difference locally, nationally, and sometimes globally," he said in a statement.
"Not only would leaving the EU see us wave goodbye to this crucial funding - but, with a smaller economy hit by new trading barriers and job losses, it's unlikely we'd be able to find that money from alternative sources.
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"Infrastructure affects the competitiveness of every business and the prosperity of every family in the country - but a Leave vote on June 23 risks putting the brakes on the infrastructure investment we need and shifting our economy into reverse."
Among projects to have benefited from EIB support, he said, were the purchase of new super express trains for the East Coast Main Line, the extension of the M8 motorway between Edinburgh and Glasgow, and the expansion of Oxford University's research and teaching facilities.
His intervention came as Jeremy Corbyn prepared to mount a scathing attack on the Conservatives as he tries to rally support among Labour voters to stay in the EU.
As the battle for votes hots up, both Mr Corbyn and Mr Cameron were out on the campaign trail for Remain - but with sharply differing messages.
The Prime Minister was keen to emphasise that the issue of Britain's membership of the EU was "bigger than party politics".
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However in a rally in London, Mr Corbyn will launch a highly partisan attack on the Conservatives, saying responsibility for many of the country's problems "lies in 10 Downing Street, not in Brussels".
His comments are likely to be seen as a response to concerns within the Remain camp that they are encountering significant hostility to the EU on the doorstep in traditional Labour areas.
The Labour leader, who for many years opposed EU membership, will highlight the importance of European regulations in underpinning workers' rights, which would be jeopardised by a Leave vote.
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"People in this country face many problems: from insecure jobs, low pay and unaffordable housing to stagnating living standards and environmental degradation, and the responsibility for them lies in 10 Downing Street, not in Brussels," he will say.
"The Tories and Ukip are on record as saying they would like to cut back our workplace rights and many unscrupulous employers would have our rights at work off us if they had the chance.
"Instead a Labour government will go further and work to raise employment standards throughout Europe to stop the undercutting of wages and strengthen every worker in Europe."
Mr Cameron said Remain campaigners from across the political spectrum were saying "with one voice" that Britain was better off in the EU.
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"This is bigger than party politics. Its effects will last longer than our lifetimes. So we are saying with one voice: make sure Britain is stronger, safer and better off - and vote to remain in a reformed European Union," he said
The official Britain Stronger In Europe campaign said that it is staging 1,000 pro-Remain events across the UK, with Liberal Democrat leader Tim Farron and the Greens' Caroline Lucas among those taking to the streets.
On the Leave side, Boris Johnson will be speaking in the South West while the Grassroots Out campaign is mounting a nationwide blitz with events across the country.
They include a rally in Chester addressed by Conservative former cabinet minister Owen Paterson and Ukip migration spokesman Steven Woolfe.
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Mr Paterson will say: "If we vote to remain, we will be consigning ourselves to being a colony of an EU Superstate, with more integration and increasingly diminished British influence.
"If we vote Leave, we will be making the safer choice, and securing the future freedom and prosperity of this region and the UK at large.
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Mr Woolfe will say: "This referendum is not about whether you are on the right of politics or on the left, whether you are Tory, Labour, Ukip or support no party at all.
"This referendum is a once-in-a-lifetime chance to take back control of our country. Unlike a general election, every vote matters."
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