Rail passengers will face further disruption on Thursday in a second round of strike action by train staff.
The industrial action is being taken by the RMT union who are locked in a dispute with ScotRail over driver-only trains.
The strike is a continuation of action that began on Tuesday and commuters are being urged to plan ahead.
ScotRail says it will still be able to run around 70% of its services as normal but insists the strikes are "totally needless" and based on misinformation.
Transport minister Humza Yousaf said: "I'm very disappointed that we're planning for a second round of industrial action when further dialogue between ScotRail and the RMT union is still an option.
"It is the travelling public that loses out in situations like this so I once again urge both parties to reopen meaningful discussions to work towards an agreement that stops further strikes from going ahead.
"I commend travellers for their efforts during the first strike earlier this week and urge them to prepare for more disruption on the railways on Thursday."
The RMT claims it has not received guarantees from ScotRail that the safety role of conductors and their role in operating train doors would not be reduced or abolished.
General secretary Mick Cash said: "The workforce know that any extension of DOO or DCO is a clear attack on our members' hard-earned terms and conditions.
"RMT members should not have to face the risk of their role and responsibilities being reduced and undermined.
"The workforce also know only too well that there is a very real threat to passengers of watering down and wiping out the safety critical role of the guard on these ScotRail services.
"That is a lethal gamble with basic rail safety."
However, ScotRail managing director Phil Verster said it was only about "who opens and closes doors on trains, nothing more".
The union will hold seven days of strikes throughout June and July.
Alternative timetables and further information on affected routes are available on the ScotRail website and passengers are being urged to check the site ahead of travelling.
Why are you making commenting on The Herald only available to subscribers?
It should have been a safe space for informed debate, somewhere for readers to discuss issues around the biggest stories of the day, but all too often the below the line comments on most websites have become bogged down by off-topic discussions and abuse.
heraldscotland.com is tackling this problem by allowing only subscribers to comment.
We are doing this to improve the experience for our loyal readers and we believe it will reduce the ability of trolls and troublemakers, who occasionally find their way onto our site, to abuse our journalists and readers. We also hope it will help the comments section fulfil its promise as a part of Scotland's conversation with itself.
We are lucky at The Herald. We are read by an informed, educated readership who can add their knowledge and insights to our stories.
That is invaluable.
We are making the subscriber-only change to support our valued readers, who tell us they don't want the site cluttered up with irrelevant comments, untruths and abuse.
In the past, the journalist’s job was to collect and distribute information to the audience. Technology means that readers can shape a discussion. We look forward to hearing from you on heraldscotland.com
Comments & Moderation
Readers’ comments: You are personally liable for the content of any comments you upload to this website, so please act responsibly. We do not pre-moderate or monitor readers’ comments appearing on our websites, but we do post-moderate in response to complaints we receive or otherwise when a potential problem comes to our attention. You can make a complaint by using the ‘report this post’ link . We may then apply our discretion under the user terms to amend or delete comments.
Post moderation is undertaken full-time 9am-6pm on weekdays, and on a part-time basis outwith those hours.
Read the rules here