The team is trying to identify a number of fair-haired men, possibly Dutch or German nationals, who were seen lurking around the apartment where the little girl was last seen in Praia da Luz, Portugal in 2007.
An appeal was broadcast on Tuesday night in Holland on a programme called Opsporing Verzocht, and was due to feature on the German programme Aktenzeichen XY...ungelost last night.
British police have already received hundreds of calls and emails after a refreshed appeal was aired on the BBC's Crimewatch on Monday night.
Madeleine's parents Kate and Glasgow-born Gerry said they are "genuinely hopeful" that a breakthrough could be possible.
Detective Chief Inspector Andy Redwood revealed that his team have now discounted a key sighting that was previously thought to be a kidnapper carrying Madeleine away from the apartment at around 9.15pm on May 3, 2007.
Instead they are looking at another man who was seen carrying a child towards the sea around 45 minutes later.
Meanwhile, the grandfather of Ben Needham, a toddler who went missing on a Greek holiday island 21 years ago, accused the Government of ignoring the case and said he feels it deserves as much support as the multi-million pound investigation into Madeleine's disappearance.
His grandfather Eddie said he feels his family has been let down.