Pickering's Gin, based in the heart of Edinburgh, will see small batches of premium spirit being distilled, bottled, labelled and sold in the city, bosses said.
The product - which contains flavourings of juniper, coriander, cardamom and fennel - is based on a recipe kept secret for more than six decades.
The gin is based on an original recipe, handwritten on a fragment of paper dated from July 17 1947, and has been tweaked to appeal to people's palates today.
The recipe resurfaced last year when the founders of the business, Matthew Gammell and Marcus Pickering, began distilling at their base at the Summerhall arts venue - the former home of the Royal (Dick) vet school.
Mr Pickering said: "The idea has been brewing for around five years and with the growing trend, popularity and resurgence of gin, now seemed the ideal time to introduce Pickering's Gin to market."
Co-founder Mr Gammell said: "We've been working on the distillery transformation for many months, and we are confident that the new small batch gin brings something new to the market, giving better choice for hotels, bars, wholesalers and consumers."
The distillery will be open for small tours and tastings on Thursday and Friday afternoons.