The Wimbledon champion bought Cromlix, three miles from his home town of Dunblane in central Scotland, in February last year.
The hotel was extensively refurbished and reopened in April to favourable reviews.
Tourism body VisitScotland has now awarded the Cromlix its top rating.
Murray said: "It's great news that Cromlix is now officially a five-star hotel.
"We've all worked hard to try and restore the hotel to its former glory and importantly, make it a nice place to go for the local community and beyond.
"We've had some great feedback so far from guests and we'll continue to make adjustments and improvements."
The Victorian mansion features 10 bedrooms and five suites and is set in 34 acres of woodland and gardens.
The hotel is being managed for the British number one by Inverlochy Castle Management International and is already fully booked for the Ryder Cup, which is being staged a short drive away at Gleneagles later this year.
References to hunting were removed from the Cromlix website earlier this month following a complaint from animal rights campaigners.
VisitScotland chairman Mike Cantlay said: "Much like Andy Murray on the tennis court, a five-star grading from VisitScotland means a hotel has reached outstanding, world-class standards across the board.
"I would like to congratulate him and his team at the Cromlix on this fantastic achievement.
"And, on behalf of everyone at VisitScotland, I would like to wish Andy the very best of luck in his title defence."