The men were named by the Serious Fraud Office (SFO) as Peter Charles Johnson, Jonathan James Mathew and Stylianos Contouglas.
They are accused of conspiracy to defraud between June 1, 2005, and August 31, 2007.
The SFO said the investigation into Libor is continuing. it announced in July 2012 it was investigating allegations of Libor-fixing.
The three men are likely to appear on February 26. at London's Westminster Magistrates' Court.
Barclays declined to comment.