The party released the figures ahead of a visit to the Forth facility by Shadow Scots Secretary Margaret Curran, who called for "honesty" from the SNP on the future of the industry in the event of a Yes vote.
"I'm looking forward to seeing the aircraft carrier under construction at Rosyth," said Ms Curran. "I'm proud that ships like this are still built in Scotland, employing thousands of men and women, and providing a boost for companies across the country.
"The contracts from the Queen Elizabeth Class aircraft carrier alone have brought over £300m worth of work to businesses across Scotland."
She added: "The people who work here deserve honesty from the SNP about the future of their industry.
"Alex Salmond wants people to believe everything will be the same after separation, but the truth is the SNP offers no guarantees for the future of these yards, the men and women who work in them, and the companies who rely on them.
"Being part of the UK offers a future for Rosyth with investment and work in the years to come."
SNP Westminster leader and defence spokesman Angus Robertson responded: "It is not the London Government that makes the yards successful. It is the second-to-none Scottish skills base and technical expertise."